Journal: Nucleic Acids Research
Article Title: The PRIDE database at 20 years: 2025 update
doi: 10.1093/nar/gkae1011
Figure Lengend Snippet: Overview of the PRIDE dataset submission, validation, storage and dissemination process. Researchers submit datasets to PRIDE Archive using the ProteomeXchange Submission Tool, which supports multiple data formats (e.g. MS raw files, processed result files, mzTab, mzIdentML, peak lists and SDRF-Proteomics). Data transfer is facilitated through services like Aspera, FTP, and the newly added Globus service. Submitted datasets are processed by PRIDE Pipelines, which perform automatic validation, submission, resubmission and publication of the private datasets. Validated datasets are stored in the PRIDE Archive, where they can be accessed via various REST APIs (e.g. PRIDE Archive Rest API, PRIDE USI Rest API, PRIDE Stream API and PRIDE Crosslinking API). These datasets are further disseminated through PRIDE’s web applications, including PRIDE Spectral Libraries, PRIDE USI and PRIDE Crosslinking, as well as external resources such as Expression Atlas, UniProt, Ensembl, OmicsDI, BioSamples and ProteomeXchange.
Article Snippet: The PRIDE Archive USI API ( https://www.ebi.ac.uk/pride/molecules/ws/swagger-ui/index.html ) allows users to retrieve specific spectra from PRIDE Archive files from Thermo Scientific instruments (see section ‘PRIDEArchive USI: Accessing and Visualizing mass spectra’).
Techniques: Expressing